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KMID : 1161420110140050454
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Journal of Medicinal Food
2011 Volume.14 No. 5 p.454 ~ p.461
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Anti-Adipogenic Activity of 2-Carbomethoxy-2,3-Epoxy-3-Prenyl-1,4-Naphthoquinone from Rubia cordifolia L.
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Jun Do-Youn
Han Cho-Rong
Lee Ji-Young
Park Wan
Choi Myung-Sook
Woo Mi-Hee
Kim Young-Ho
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Abstract
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To examine anti-adipogenic activity of 2-carbomethoxy-2,3-epoxy-3-prenyl-1,4-naphthoquinone (CMEP-NQ) isolated from the roots of Rubia cordifolia L., its effects on cell viability, apoptosis, and adipogenesis in 3T3-L1 preadipocytes were investigated. The inhibitory effect of CMEP-NQ on cell viability was more significant in differentiated mature adipocytes than in 3T3-L1 preadipocytes. In 3T3-L1 cells, the cytotoxicity of CMEP-NQ (20?40?μM) was accompanied by apoptotic events such as mitochondrial membrane potential loss, caspase-3 activation, poly(ADP-ribose) polymerase degradation, and terminal deoxynucleotidyl transferase-meidated dUTP nick-end labeling?positive apoptotic DNA fragmentation. Although the presence of 10?μM CMEP-NQ during induced adipocytic differentiation of 3T3-L1 cells for 6 days failed to influence the cell viability, it did reduce the differentiation-associated accumulation of intracellular lipid by approximately 48.5%. A similar level of inhibition was observed when 10?μM CMEP-NQ was present during the early stage (Days 0?2) of the differentiation period. At the same time, the expressions of CCAAT/enhancer binding protein-α, peroxisome proliferator-activated receptor γ1, peroxisome proliferator-activated receptor γ2, and adiponectin were down-regulated. However, the presence of 10?μM CMEP-NQ during either the middle (Days 2?4) or late (Days 4?6) stage of the differentiation period caused the inhibition to a lesser extent. These results indicated that CMEP-NQ at high concentrations (20?40?μM) exerted cytotoxicity via inducing apoptosis, whereas CMEP-NQ at a low concentration (10?μM) suppressed adipocytic differentiation without exerting cytotoxicity in 3T3-L1 preadipocytes.
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KEYWORD
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3T3-L1 preadipocyte, anti-adipogenesis, apoptosis, 2-carbomethoxy-2,3-epoxy-3-prenyl-1,4-naphthoquinone, CCAAT/enhancer binding protein-α, peroxisome proliferator-activated receptor γ1, peroxisome proliferator-activated receptor γ2, Rubia cordifolia L.
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